Vol.1 (2), October 2016

Determination of pesticide residues in bee produces honey using gas chromatography mass spectrometry

Original Research Article

Taye Beyene*, Mekonen Woldatsadik and Desta Abi

Adami Tulu Agricultural Research Center P.O.Box 35, Zeway, Ethiopia

date Received: September 10, 2016     date Accepted: October 14, 2016     date Published: October 30, 2016


Increasing emissions of chemical compounds to the environment, especially of pesticides, is one of the factors that may explain present honeybee colony loses. The widespread and careless use of toxic pesticides during the blooming periods of agricultural and horticultural crops not only kills honeybees but also contaminated hive products. For this reason the analysis of pesticide residues in honey helps to assess potential risk of this product to consumer health and gives information on the pesticide treatments that has been in field crops surrounding apiary site. The study was conducted in West Arsi and East Shoa Zones, Oromia Region of Ethiopia with the objective of determining the presence  of pesticide residues in honey samples and its conformity with to determine whether it is within the limits of European Union range of Maximum Residue with Levels (MRLs). For this study a total of eighteen locally bee produce honey samples (six apiaries; three samples from each apiary) were collected directly from beekeepers just after harvesting from the selected districts namely Dugda, Arsi Negele and Sheshamene. The samples were analyzed based on solid-phase extraction with octadecyl sorbent followed by gas chromatography mass spectrometry (GC-MS). The result obtained from the study revealed that none of the targeted pesticide compounds, which included organochlorine, organophosphorus, pyrethroids and aryloxyalkanoic acid appeared in samples or exceeded 0.001mg/kg detection level. Therefore, honey produce in mid rift valley of Ethiopia are free from pesticide residues and safe for human consumption. Although the study results showed none significant pesticide residue in the analyzed honey samples, a special precaution should be taken regarding to production of pesticides, their sale, and application in the future.



Key words:

Key words: Gas chromatography, honey, honeybees, pesticide, residues

Determination of pesticide residues in bee produces honey using gas chromatography mass spectrometry